INTRATESTICULAR SUBCAPSULAR ASSAY, A SIMPLE METHOD FOR THE PRELIMINARY SCREENING OF MALE CONTRACEPTIVES

The MB-50 protocol worked out for long by the Worm Health Organization has been widely employed for the screening of male contraceptives. With this method, 40～80 rats will be used for 1 sample and the course of study will be about 3 months. In view of the high expenses and long duration of time needed, it seems to be not suitable for a preliminary screening. We have exploited a much simpler method, the intratesticular subcapsular assay. The method is as follows: Puncture the scrotal skin and the tunica albuginea at the equatorial plane of the testis just opposite to the epididymis with an intradermal needle fixed at a tuberculin syringe. Then push on the needle for 3～4 mm along the equator parallel to the tunica albuginea. Inject 100 μl of solution containing 0.1～5 mg of the sample to each testis. To the controls, only the vehicle is injected. One week later the same procedure is repeated and 2 more weeks later, the animals are sacrificed and the data are observed (see Table 1 and 2). In this paper, 7 samples screened with this method were compared with results of the MB-50 method, no positive sample was missed except one false positive sample. These results indicate the coincidence between these two methods.