Determination of isosteroidal alkaloids and glucosides in Fritillaria by HPLC-ELSDSimultaneous determination of the major isosteroidal alkaloids and their glucosides in the bulbs of Fritillaria by high performance liquid chromatography coupl

AimTo establish an HPLC-ELSD method for the simultaneous determination of five major bioactive isosteroidal alkaloids and gluco-alkaloids in the bulbs of Fritillaria namely peimissine, imperialine, sinpeinine　A, imperialine-3β-glucoside and yibeinoside A. MethodsA Nova-Pak C₁₈ column (150 mm×3.9 mm ID) was used. The chromatography was carried out with a linear gradient programming. The mobile phase was acetonitrile-water (containing 0.1% diethylamine) and the flow rate was 1.0 mL·min^-1. ResultsThe linear range of peimissine was 13.1-288.2 mg·L^-1 (r²=0.997 5), imperialine-3β-glucoside 7.7-169.4 mg·L^-1 (r²=0.999 3), yibeinoside A 7.3-160.6 mg·L^-1 (r²=0.999　7), imperialine 16.5-363.0 mg·L^-1 (r²=0.999　2), sinpeinine A 8.7-191.4 mg·L^-1 (r²=0.994　2). ConclusionThe method is accurate with overall intra- and inter-day variation less than 5% and recovery more than 95%. The method was successfully applied to analyze five major bioactive alkaloids and gluco-alkaloids in three Fritillaria bulbs.