Parallel catalytic hydrogen wave of acetylspiramycin caused by dissolved oxygen and its application

AimTo propose a novel polarographic method for the determination of acetylspiramycin (ASPM) is proposed. MethodsIn 0.1 mol·L^-1 NH₄Cl-NH₃·H₂O (pH 8.9) buffer containing dissolved oxygen, ASPM yielded a sensitive parallel catalytic hydrogen wave with the peak potential of -1.63 V (vs SCE) by single sweep polarography. ResultsThe 2nd order derivative peak currents (i_p″) of the parallel catalytic hydrogen waves of ASPM showed a linear relationship with its concentrations in the range from 1.74×10^-3 μg·mL^-1 to 3.84 μg·mL^-1 (r=0.997 9, n=13). Its detection limit was 5.80×10^-4 μg·mL^-1 (3σ) and RSD (n=13) was 1.24% at the concentration level of 0.871 μg·mL^-1. ConclusionThe proposed method could be applied to the determination of ASPM in ASPM tablets.