SEPARATION AND QUANTITATIVE DETERMINATION OF BAICALIN AND CHLOROGENIC ACID IN SHUANGHUANGLIAN ORAL LIQUID BY HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS

AIM: To establish a high performance capillary electrophoresis (HPCE) method for the separation and quantitative determination of baicalin and chlorogenic acid in Shuanghuanglian oral liquid. METHODS: Based on the mode of capillary zone electrophoresis, baicalin and chlorogenic acid were separated successfully from other components and determined within 14 minutes under the optimum electrophoretic conditions as follows:uncoated fused silica capillary (34 8 cm×39 5 cm, 50 μm ID), 40 mmol·L^-1 borax (pH 9.00 for baicalin and pH 9.55 for chlorogenic acid), applied voltage 17 kV, UV 285 nm for baicalin and 326 nm for chlorogenic acid, pressure injection 68 95 kPa·s. RESULTS: The relative peak areas of the analytes increase linearly with the increase of their concentrations in the range of 20～640 μg·mL^-1 for baicalin and 8～400 μg·mL^-1 for chlorogenic acid. Their recoveries are 100.60%±2.36% and 99.68%±2.27% respectively. CONCLUSION: The analytical results demonstrate that the method is convenient, rapid and well reproducible, and can be used as a reliable tool for the quality control of this prescription.