Two new flavonoids from the flower of Castanea mollissima Blume

AimTo study the bioactive constituents from the flower of Castanea mollissima Blume． MethodsCompounds were isolated and purified by column chromatography on silica gel and polyamide．Structures were determined by various spectroscopic data，including UV， IR， ¹H and ₁₃CNMR， EIMS and FABMS， ¹H-₁₃C-COSY and HMBC． ResultsTwo compounds were isolated from the ethyl acetate fraction of 95% ethanol extract and the structures were elucidated as kaempferol-3-O-［6″-(E)-P-coumaroyl］-α-D-mannopyranoside (1) and kaempferol-3-O-［6″，4″-di-(E)-P-coumaroyl］-α-D-mannopyranoside (2)． ConclusionCompounds 1 and 2 are new compounds，named castanoside A and B respectirely．