Isolation and structure identification of C-25 epimers of inokosterone from Achyranthes bidentata Blume.

AimTo isolate C-25 epimers of inokosterone from Achyranthes bidentata Blume. and identify their structures. MethodsTo separate C-25 epimers of inokosterone by using various kinds of chromatography methods and identify their structures on basis of spectral analysis and chemical method. ResultsThree compounds were isolated and their structures were established as 25S-inokosterone (1), 25R-inokosterone (2) and ecdysterone (3). ConclusionCompounds 1 and 2 are new C-25 configuration isomers from Achyranthes bidentata Blume., their absolute configurations are elucidated at the first time, and their ¹³CNMR data are reported for the first time.