THE EFFECT OF HARRINGTONINE ON THE CELL CYCLE OF L-1210 CELLS AND THE BONE MARROW STEM CELLS IN MICE
-
Abstract
By use of autoradiographic method the duration of the cell cycle of L-1210 leukemic cells and the effect of harringtonine on the cell cycle of the leukemic cells in mice were studied. The TC of L-1210 cells on the 6th day after transplantation was 15.8 hours, and the TS, TG1, TG2, TM were 10.7, 2.0, 2.7, 0.4 hours, respectively. Under the action of harringtonine the duration of S phase of the leukemic cells was prolonged and the mitotic index was lowered after 5 hours of the administration of the drug. The labelled index of the cell population and the average grain counts per cell by the 3H-TdR of the treated leukemic cells were lowered significantly. These results. suggest that the DNA synthesis was inhibited profoundly after the treatment with harringtonine.By means of the spleen colony assay technique the action of harringtonine on normal bone marrow stem cells was investigated. It was revealed that when the dosage of harringtonine was 1owet than 0.5 mg/kg no effect on the stem cells was found, whilea dosage dependent, effect on the survival rate of colony-forming unit in spleen was. noted when the dosage was. more than 0.5 mg/kg. It is interesting that the total saponine of Panax Ginseng was found to protect the stem cells of bone marrow in micefrom the killing effect of harringtonine.
-
-
DownLoad: