Suppressive effect in vitro of resveratrol on ADP induced human platelet aggregation and its active mechanism

Resveratrol (RESV) is a polyphenolic compound existed in native plants such as grape, fleeceflower root, and peanut, etc. The aim of this study was to investigate the effects in vitro of RESV on adenosine diphosphate (ADP)-induced platelet aggregation, platelet membrane-bound fibrinogen (PFig) its mechanism of action. The effects of RESV and phospholipase Cβ inhibitor (U73122) on ADP-induced healthy human volunteers platelet aggregation, PFig, and the expression of phospho-phospholipase Cβ3 (P-PLCβ3) and total-phospholipase Cβ3 (T-PLCβ3) were studied with platelet aggregometer, flow cytometry and Western blotting, respectively. Compared with control group, RESV at 25, 50 and 100 μmol·L^-1 inhibited ADP-induced platelet aggregation and PFig in a dose dependent manner，and RESV at 25 μmol·L^-1 obviously reduced expression of P-PLCβ3 and ratio of P-PLCβ3 to T-PLCβ3 in platelet of healthy human volunteers. Furthermore, RESV and U73122 had additive effect in inhibiting platelet aggregation and PFig. All these suggested that RESV inhibited platelet aggregation and PFig induced by ADP partly through decreasing the activity of PLCβ of platelets, and that RESV had definite effect of antiplatelet and might be developed as a novel antithrombotic agent.