Flow injection chemiluminescent detection of acemetacin in KMnO₄-Na₂SO₃system

AimTo study the sensitizing effect of acemetacin (ACE) on the weak chemiluminescent (CL) reaction of KMnO₄ with sulfite and establish a fast and convenient method for CL detection of ACE. MethodsUsing the sensitizing effect of ACE on KMnO₄-Na₂SO₃ system and flow injection technique to determine the concentration of ACE. ResultsUnder optimal conditions, the CL intensity of 1.0×10^-2 mol·L^-1 H₃PO₄ - 5.0×10^-5 mol·L^-1 KMnO₄ - 4.0×10^-4mol·L^-1 Na₂SO₃ was proportional to the concentration of ACE ranging from 1.0×10^-7 to 1.0×10^-5 mol·L^-1. The detection limit of ACE was 6.9×10^-8 mol·L^-1 at 3σ. Satisfactory results were obtained for determination of ACE at 2.5×10^-6 mol·L^-1. ConclusionThe present method showed good precision，high sensitivity and selectivity and could be used for fast and convenient detection of ACE. It would be of significance to the clinical and pharmacological study of acemetacin.