DETERMINATION OF METHAQUALONE AND ITS METABOLITES IN URINE AND BLOOD BY UV,GC/FID AND GC/MS

A systematic procedure for the determination of methaqualone and its metabolitesin blood and urine by UV spectrophotometry ,GC and GC/ MS was developed.Urine and bloodsamples were from a suicidal patient who ingested 18 tablets of methaqualone. Both solid phase andliquid-liquid extractions were used in the extraction and clean-up of the samples.The total amount ofmethaqualone and its metabolites was measured by UV spectrophotometry.The amount of parentmethaquaione was quantitated by GC/FID,Methaqualone and its 10 metabolites including two acetylmetabolites were found in urine and bloed。 This procedure is useful for monitoring drugs in emergencytreatment.