ISOLATION AND STRUCTURE IDENTIFICATION OF LIGAN GLYCOSIDES FROM PINE NEEDLES OF PINUS MASSONIANA LAMB.

AIMTo study the chemical constituents of the pine needles of PINUS MASSONIANA lamb.. METHODSVarious chromatographic techniques were used to separate and purify. Their physico-chemical properties and spectral data (UV, IR, MS, ¹H-¹H COSY, HMQC, DEPT, HMBC and ORD ect.) were measured for structure elucication. RESULTSThree compounds were isolated from the n-BuOH fraction of water-extracts. Their structures were identified as massonianoside A (4), massonianoside A: (7S,8R)-3,4,9′-trihydroxyl-3-methyoxyl-7,8-dihydrobenzofunan-1′-propanolneoligan-9-o-α-L-rhamnopyranoside, massonianoside C (5), (7S,8R)-9,9′-dihydroxyl-3,3′-dimethyoxyl-7,8-dihydrobenzofunan-1′-propanolneoligan-4-o-α-L-rhamnopyranoside and cedrusin-4-o-β-glucoside (6), (7S,8R)-3′,9,9′-trihydroxyl-3-methoxyl-7,8-dihydrobenzofunan-1′-propanolneoligan-4-o-β-D-glucopyranoside. CONCLUSIONCompound 4 and 5 are new compounds.