STUDIES ON THE ACTIVE CONSTlTUENTS OF THE CHlNESE TRADlTIONAL M EDlCINE POLYGONATUM ODORATUM (MILL．)DRUCE

From the ethanol extract of the Chinese traditional medicine ,rhizomes of Polygona-tum odoratum,a new steroidal saponin POD-Ⅱ together with β-sitosterol and its glucoside ,and threeknown saponins were isolated.On the basis of chemical evidence and spectral analysis(UV ,IR,EI-MS,¹HNMR and1 ³CNMR),the structure of POD-Ⅱ was elucidated as 3-O-β-D-glucopyranosyL-(1→2)- β-D- xylopyranosyl-( 1→3)- β-D- glucopyranosyl-(1→4)-galactopyranosyl-25(R)- spirost-5- en-3β,14α-diol.Preliminary pharmacologicaI tests showed that POD-Ⅱ could induce the colony stimu-lating factor(CSF)in mouse serum; and POD-Ⅲ,at the concentration of 0.1μg·mL^-1 was shown tosynergize the suboptimal and optimal concentration of Con A and lipopolysaccharide to stimulate lymp-hocytes proliferation.